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Diagnostic Strategies
  1. To confirm a Giardiasis infection
    If one has to rely on microscopy, at least three independent stool samples should be screened before one rules out the diagnosis! As an alternative strategy, one could use an antigen detection assay analysing one sample. If the test result is negative a second sample should be screened.
  2. To screen patients with diarrhoea for protozoa infections
    At least three stool samples should be taken. Microscopic analyses allow detection of several pathogens. This may also be realized using a multiple antigen detection assay (e.g. Triage Micro Parasite assay). In the near future, a multiplex rt-PCR might be the optimized method, depending on its validation.
  3. For epidemiological studies
    For convenience one may rely on antigen detection, avoiding laborious microscopy.