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Diagnostic Methods
Diagnostic methodsPros and cons
Parasitological methods 
Diagnosis is based on finding eggs in stools or in bile aspirated from the duodenum.
Passage of eggs can be detected temporarily after a meal with infected liver!
The diagnosis can be confined by serodiagnostic tests when available.
  • Parasitological diagnosis is not possible during the long prepatent period
  • Sensitivity restricted by variation in egg production (multiple specimens needed)
  • Differentiation of Fasciola species difficult due to intraspecific morphological variations of ova
Molecular diagnosis 
A PCR-RFLP assay has been developed for the distinction between F. hepatica and F. gigantica. 
Antigen detection 
No tests developed so far 
Antibody detection 
The newer serological assays (mainly ELISA formats) use recombinant or purified antigen (e.g. cathepsin L1)
  • Sensitivity is high
  • Specificity is limited